|
KMID : 0043320080310081004
|
|
Archives of Pharmacal Research
2008 Volume.31 No. 8 p.1004 ~ p.1009
|
|
|
Inhibition of Skin Inflammation and Atopic Dermatitis by Topical Application of a Novel Ceramide Derivative, K112PC-5, in Mice
|
|
Kang Jong-Soon
Youm Jong-Kyung
Park Byeong-Deog
Han Sang-Bae
Lee Chang-Woo
Jeong Se-Kyoo
Kim Hwan-Mook
Park Song-Kyu
Han Gyoon-Hee
Lee Ki-Ho
Han Mi-Hwa
Lee Hyun-Ju
|
|
|
Abstract
|
|
|
|
PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide) is a synthetic ceramide and has been known to be effective in atopic and psoriatic patients. K112PC-5 (2-Acetyl-N-(1,3-dihydroxyisopropyl)-tetradecanamide) is a novel ceramide derivative of PC-9S. In the present study, we examined the effect of K112PC-5 on macrophage and T lymphocyte function in primary macrophages and splenocytes, respectively, as well as the effect of topical application of K112PC-5 on skin inflammation and atopic dermatitis (AD) in mouse models. K112PC-5 inhibited lipopolysaccharide-induced nitrite generation in mouse peritoneal macrophages in a dose-dependent manner. However, K112PC-5 did not affect concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In addition, K112PC-5 significantly suppressed the increase in phorbol ester-induced ear thickness in BALB/c mice. Further study demonstrated that topical application of K112PC-5 also inhibited AD induced by extracts of dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, in NC/Nga mice. Taken together, these results showed that K112PC-5 exerted an anti-inflammatory effect both in vitro and in vivo and proved to be beneficial in an animal model of AD. Our results suggest that K112PC-5 might be beneficial as a topical agent for the treatment of AD.
|
|
|
KEYWORD
|
|
|
K112PC-5, Macrophage, Skin inflammation, Atopic dermatitis, Dust mite, NC/Nga
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|